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Updated: May 12, 2026

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
Published on: July 22, 2008
Polycomb complex recruitment in pluripotent stem cells
Maria J Barrero1, Juan Carlos Izpisua Belmonte
1Center of Regenerative Medicine in Barcelona, Aiguader 88, E-08003 Barcelona, Spain.
Abstract:
The recruitment of the silencing complex Polycomb group (PcG) to its target sites in mammalian cells has remained elusive. A prevalent model proposes that the PRC1 component is recruited through recognition of methylated H3K27 found at target sites occupied by the PRC2 component. However, mounting evidence suggests that PRC2-independent mechanisms of PRC1 recruitment exist. Three studies describe that the histone demethylase Kdm2b binds to unmethylated CpG islands and recruits a subset of PRC1 complexes to chromatin in pluripotent stem cells.
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