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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
Published on: October 21, 2022
Circulating miRNA profile in HCV infected serum: novel insight into pathogenesis
Shivaprasad Shwetha1, K Gouthamchandra, Madhavi Chandra
1Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India.
Scientific Reports
|April 4, 2013
Summary
Circulating microRNAs (miRNAs) show altered levels in Hepatitis C Virus (HCV) infection. Specific miRNAs like miR-134, miR-320c, and miR-483-5p are upregulated, offering insights into HCV pathogenesis.
Area of Science:
- Biochemistry
- Molecular Biology
- Virology
Background:
- Circulating microRNA (miRNA) profiles are altered in various diseases.
- Hepatitis C Virus (HCV) infection impacts host cellular processes.
Purpose of the Study:
- To profile circulating miRNAs in serum from HCV-infected individuals.
- To identify specific miRNAs associated with HCV infection across different genotypes.
- To explore the role of these miRNAs in HCV pathogenesis.
Main Methods:
- Utilized a microRNA array to profile 940 miRNAs in serum from HCV genotypes 1 and 3 infected individuals and healthy controls.
- Validated upregulated miRNAs (miR-134, miR-198, miR-320c, miR-483-5p) in 36 patient samples.
- Assessed miRNA expression in HCV-JFH1 infected cells and supernatant.
Main Results:
- Identified significant upregulation of serum miR-134, miR-320c, and miR-483-5p in HCV infection.
- Observed upregulation of miR-320c and miR-483-5p in HCV-JFH1 infected cells and supernatant.
- Pathway analysis implicated PI3K-Akt, NFKB, and MAPK signaling in miRNA target genes.
Conclusions:
- Circulating miR-134, miR-320c, and miR-483-5p are potential biomarkers for HCV infection.
- These miRNAs may play a role in the pathogenesis of HCV by influencing key signaling pathways.
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