Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Computer-aided sperm analysis: past, present and future.

J C Lu1, Y F Huang, N Q Lü

  • 1Department of Laboratory Science, Nanjing Hospital, Jiangsu Corps, The Armed Police Force, PLA, Nanjing, China.

Andrologia
|April 5, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[The impact of coronary artery calcification on the long-term outcomes after chronic total occlusion percutaneous coronary intervention].

Zhonghua xin xue guan bing za zhi·2025
Same author

Clinical prediction models for in vitro fertilization outcomes: a systematic review, meta-analysis, and external validation.

Human reproduction (Oxford, England)·2025
Same author

[Early cellular immune exhaustion in patients with Epstein-Barr virus activation following haploidentical hematopoietic stem cell transplantation].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

[A case of capsular bag necrosis syndrome].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2024
Same author

[Efficacy comparison of the S8 navigation-assisted correction versus manual correction in adolescent congenital scoliosis].

Zhonghua yi xue za zhi·2024
Same author

[Research advances on the role of histone modification in cardiac aging and related vascular diseases].

Zhonghua xin xue guan bing za zhi·2024
Same journal

Correction.

Andrologia·2023
Same journal

The combination of ginger and zinc supplement could improve lead-induced reproductive dysfunction by inhibiting apoptosis mediated by oxidative damage and inflammation.

Andrologia·2022
Same journal

Testosterone and lifespan in males: At the right time and the right level.

Andrologia·2022
Same journal

CORRIGENDUM.

Andrologia·2022
Same journal

CORRIGENDUM.

Andrologia·2022
Same journal

Microfluidic devices employing chemo- and thermotaxis for sperm selection can improve sperm parameters and function in patients with high DNA fragmentation.

Andrologia·2022
See all related articles

Computer-aided sperm analysis (CASA) systems are widely used but lack of technician knowledge and quality control leads to errors. Standardization and quality control are essential for accurate CASA results in clinical laboratories.

Area of Science:

  • Andrology
  • Medical Laboratory Science
  • Reproductive Medicine

Background:

  • Computer-aided sperm analysis (CASA) is a standard tool in clinical laboratories for semen analysis.
  • Technicians often lack comprehensive understanding of CASA principles and potential error sources.
  • Variability in CASA results between systems and labs necessitates improved quality control.

Purpose of the Study:

  • To review the fundamental concepts and technological evolution of CASA systems.
  • To discuss the clinical applications and limitations of CASA technology.
  • To identify key factors affecting CASA accuracy and recommend standardization measures.

Main Methods:

  • Literature review focusing on CASA principles, development, and clinical use.
Keywords:
Clinical applicationcomputer-aided sperm analysisinfluencing factorquality controlstandardisation

More Related Videos

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
05:44

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

Related Experiment Videos

Last Updated: May 12, 2026

Fish Sperm Assessment Using Software and Cooling Devices
07:57

Fish Sperm Assessment Using Software and Cooling Devices

Published on: July 28, 2018

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
08:48

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
05:44

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Published on: March 1, 2019

  • Analysis of factors influencing CASA accuracy, including frame rate, counting chambers, algorithms, and sperm concentration.
  • Discussion on the impact of quality control and standardization.

    • Main Results:

    • CASA systems are prevalent but susceptible to errors due to technical factors and insufficient quality control.
    • Factors like frame rate, counting chambers, algorithms, and sperm concentration significantly impact result accuracy.
    • Significant inter-system and inter-laboratory variability exists in CASA outcomes.

    Conclusions:

    • Standardization and rigorous quality control are crucial for improving the reliability of CASA systems.
    • Enhanced technician training on CASA principles and error sources is necessary.
    • Addressing identified factors can minimize errors and enhance diagnostic accuracy in semen analysis.