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Updated: May 12, 2026
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
Retinal and choroidal intravascular spectral-domain optical coherence tomography
Anne Willerslev1, Xiao Q Li, Peter Cordtz
1Department of Ophthalmology, Glostrup Hospital, Glostrup, DenmarkFaculty of Health Sciences, University of Copenhagen, Copenhagen, DenmarkDepartment of Ophthalmology, Roskilde Hospital, Roskilde, Denmark.
Purpose:
To examine retinal and choroidal blood vessels using spectral-domain optical coherence tomography (SD-OCT).
Methods:
Retrospective case series.
Results:
Scans through retinal blood vessels in healthy subjects demonstrated vessel wall reflexes and a tri-layer profile of the blood column on longitudinal scans and a figure-of-eight configuration on cross-sectional scans. Intravascular reflectivity decreased with increasingly oblique angles of observation and was absent when blood flow was parallel to the line of sight. The high blood flow in the choroidal vessels in healthy subjects and the low flow in the retinal vessels in patients with ocular ischaemic syndrome and central retinal artery occlusion were both associated with lower reflectivity of the blood and an unstructured intravascular SD-OCT profile.
Discussion:
This qualitative in vivo study found a characteristically structured SD-OCT profile of the blood column in retinal vessels with normal blood flow. Both structure and total reflectivity faded when blood flow was lower or higher than normal or at oblique angles to the line of sight. In conclusion, SD-OCT scans of the vessels in the posterior pole of the eye may assist the clinical assessment of gross abnormalities of ocular blood flow, e.g. in carotid artery stenosis.
