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Related Experiment Video

Updated: May 12, 2026

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

Three-dimensional deconvolution microfluidic microscopy using a tilted channel.

Nicolas C Pégard¹, Jason W Fleischer

¹Princeton University, Department of Electrical Engineering, OldenStreet, Princeton, New Jersey 08544, USA.

Journal of Biomedical Optics

|April 5, 2013

Summary

This summary is machine-generated.

We created a microfluidic device for 3D imaging of flowing cells. This technology provides detailed volume and surface information, improving cell analysis in microscopy and flow cytometry.

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Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

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Published on: September 9, 2022

Area of Science:

Biomedical Engineering
Microfluidics
Optical Imaging

Background:

Accurate 3D imaging of flowing biological samples is crucial for cell analysis.
Traditional microscopy methods often require complex setups for 3D reconstruction.

Purpose of the Study:

To develop a novel microfluidic device for efficient 3D imaging of dynamic samples.
To enable high-throughput, non-invasive 3D profiling of cells in a liquid environment.

Main Methods:

A tilted microfluidic channel was designed to capture a focal stack of flowing samples.
Computational deconvolution algorithms were applied to the focal stack to reconstruct 3D images.
The device was tested using flowing yeast cells.

Main Results:

The microfluidic device successfully generated 3D images of yeast cells.
Both cellular volume and surface profile information were obtained.
The system demonstrated high sample throughput and operated in a controlled liquid environment.

Conclusions:

The developed microfluidic device offers a simplified and efficient approach to 3D imaging.
It integrates seamlessly with existing microscopy and flow cytometry platforms.
This technology has significant potential for advanced cell analysis and diagnostics.