Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)
07:46

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

Published on: January 22, 2022

Axonal excitability during ischemia in MELAS.

Karl Ng1, Kishore R Kumar, Carolyn Sue

  • 1Department of Neurology and Clinical Neurophysiology, Royal North Shore Hospital, New South Wales, Australia. kng@med.usyd.edu.au

Muscle & Nerve
|April 5, 2013
PubMed
Summary

Researchers investigated axonal excitability in patients with mitochondrial myopathy, lactic acidosis, and stroke-like episodes (MELAS) under ischemic stress. No significant differences in axonal responses were found compared to controls, suggesting distinct neuropathy mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Parkinson's disease genetics across diverse ancestries: an observational genetic study of causal and risk variants with translational implications.

The Lancet. Neurology·2026
Same author

Targeted long-read sequencing enables comprehensive analysis of the genetic and epigenetic landscape of inherited myopathies.

Nature communications·2026
Same author

<i>GCH1</i> p.Ser80Asn Confers Risk for Parkinson's Disease in East Asian Populations.

medRxiv : the preprint server for health sciences·2026
Same author

Reduced electroencephalogram power and no change in peak alpha frequency in individuals with chronic migraine: a cross-sectional investigation.

Neurophysiologie clinique = Clinical neurophysiology·2026
Same author

Monoallelic POLR3A Variants Cause Early-Onset Peripheral Neuropathy.

Annals of neurology·2026
Same author

Optical mapping reveals a higher level of large-scale structural variants in a family with paternally transmitted myotonic dystrophy and independent Parkinson's disease.

The Journal of pathology·2026

Area of Science:

  • Neuroscience
  • Mitochondrial Biology
  • Neurology

Background:

  • Mitochondrial diseases like MELAS may cause energy substrate depletion, leading to ATPase-dependent pump dysfunction and membrane depolarization.
  • Axonal depolarization is observed during crises in MELAS but not typically in resting states.
  • Ischemia is used to stress axons and reveal abnormal responses, similar to observations in diabetic neuropathy.

Purpose of the Study:

  • To investigate axonal excitability in patients with MELAS under ischemic stress.
  • To determine if ischemia reveals abnormal axonal responses related to ATP-dependent mechanisms in MELAS.
  • To compare axonal responses in MELAS to those observed in diabetic neuropathy.

Main Methods:

  • Studied excitability of median nerve axons in 13 MELAS patients (6 with glucose intolerance) and 17 controls.

More Related Videos

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
06:55

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Published on: August 4, 2022

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke
07:00

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Published on: February 4, 2014

Related Experiment Videos

Last Updated: May 12, 2026

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)
07:46

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

Published on: January 22, 2022

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
06:55

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Published on: August 4, 2022

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke
07:00

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Published on: February 4, 2014

  • Applied ischemia to the arm for 10 minutes by inflating a cuff.
  • Measured axonal excitability before, during, and after ischemia.

    • Main Results:

    • No significant differences were found in pre-ischemic axonal excitability between MELAS patients and controls.
    • Intra-ischemic and post-ischemic axonal responses did not differ significantly between the groups.
    • The study failed to demonstrate a defect in axonal ATP-dependent mechanisms under ischemic stress in MELAS.

    Conclusions:

    • Axonal ATP-dependent mechanisms do not appear to be detectably impaired under ischemic stress in MELAS.
    • The observed axonal depolarization during MELAS crises may involve different mechanisms than those revealed by ischemia.
    • Neuropathy mechanisms in MELAS may differ from those implicated in diabetic neuropathy.