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Updated: May 12, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
Separation of nonfucosylated antibodies with immobilized FcγRIII receptors
Glen R Bolton1, Margaret E Ackerman, Austin W Boesch
1Process Biochemistry, Biogen Idec, Cambridge, MA, 02142, USA.
Abstract:
Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity.
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