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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Catalyzing plant science research with RNA-seq.

Laetitia B B Martin1, Zhangjun Fei, James J Giovannoni

  • 1Department of Plant Biology, Cornell University Ithaca, NY, USA.

Frontiers in Plant Science
|April 5, 2013
PubMed
Summary

RNA-sequencing (RNA-seq) offers rapid, high-resolution analysis of plant transcriptomes. This powerful technology aids in understanding plant biology, gene networks, and environmental responses across various applications.

Keywords:
RNA-seqnext generation sequencingplant transcriptomesystems biologytranscriptomics

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Area of Science:

  • Plant Biology
  • Genomics
  • Molecular Biology

Background:

  • Next-generation DNA sequencing enables advanced plant transcriptome analysis via complementary DNA (cDNA) sequencing, known as RNA-sequencing (RNA-seq).
  • RNA-seq has emerged as a versatile tool for whole genome profiling, offering high resolution and affordability.
  • Its applications span diverse areas, from cellular-level studies to evolutionary insights in plant biology.

Purpose of the Study:

  • To provide an overview of the diverse applications of RNA-sequencing in plant biology.
  • To summarize the range of RNA-seq protocols developed for specific research questions.
  • To highlight RNA-seq's role in advancing systems biology approaches.

Main Methods:

  • Sequencing of complementary DNA (cDNA) populations derived from plant transcriptomes.
  • Application of RNA-sequencing (RNA-seq) protocols for high-resolution genomic analysis.
  • Utilizing diverse RNA-seq methodologies for cell type-specific, structural, and functional transcriptomic studies.

Main Results:

  • RNA-seq facilitates genomic data generation for unsequenced species, expanding the scope of model organisms.
  • It elucidates plant gene regulatory networks and responses to developmental and environmental cues.
  • RNA-seq integrates transcriptomics, proteomics, and metabolomics within a systems biology framework.

Conclusions:

  • RNA-sequencing is a transformative technology in plant biology, enabling detailed molecular and evolutionary studies.
  • The versatility of RNA-seq protocols allows for tailored investigations into complex biological questions.
  • RNA-seq significantly advances our understanding of plant systems biology and gene function.