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Resveratrol prevents interleukin-1β-induced dysfunction of pancreatic β-cells

Fang Chen1, Xiaohua Zhou, Yan Lin

  • 1Key Laboratory of Human Functional Genomics of Jiangsu Province, Clinical Diabetes Centre of Jiangsu Province, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

Journal of Biomedical Research
|April 5, 2013
PubMed
Abstract

Insights

Resveratrol protects pancreatic beta cells from Interleukin-1β (IL-1β) induced dysfunction. This polyphenol reduces nitric oxide production and preserves insulin secretion, offering a potential therapeutic strategy for diabetes.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Pharmacology

Background:

  • Interleukin-1β (IL-1β) is implicated in the pathogenesis of type 1 and type 2 diabetes mellitus.
  • Resveratrol, a naturally occurring polyphenol, exhibits diverse pharmacological activities.
  • IL-1β contributes to pancreatic beta-cell dysfunction, a key factor in diabetes development.

Purpose of the Study:

  • To investigate the protective effects of resveratrol against IL-1β-induced pancreatic beta-cell dysfunction.
  • To elucidate the molecular mechanisms underlying resveratrol's action on beta cells exposed to IL-1β.

Main Methods:

  • Assessed nitric oxide (NO) production using the Griess assay.
  • Measured peroxisome proliferator-activated receptor-γ (PPAR-γ) transcriptional activity via reporter assays.
  • Evaluated inducible nitric oxide synthase (iNOS) expression and nuclear factor-κB (NF-κB) activation using Western blotting and electrophoretic mobility shift assays (EMSA).
  • Determined the impact on glucose-stimulated insulin secretion in isolated rat pancreatic islets.

Main Results:

  • Resveratrol significantly reduced IL-1β-induced NO production, correlating with decreased iNOS mRNA and protein levels.
  • The protective effect involved enhanced PPAR-γ activity, leading to the inhibition of NF-κB activation.
  • Resveratrol prevented IL-1β-mediated impairment of glucose-stimulated insulin secretion in rat islets.

Conclusions:

  • Resveratrol demonstrates a protective role against IL-1β-induced pancreatic beta-cell dysfunction.
  • The findings suggest resveratrol's potential as a therapeutic agent for managing diabetes-related beta-cell damage.
Keywords:
interleukin-1βnitric oxidenuclear factor-κB.peroxisome proliferator-activated receptor-γresveratrol

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