Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Time-resolved IR and X-ray spectroscopy as complementary electronic structure probes of photoinduced ligand-exchange in molybdenum hexacarbonyl.

Chemical communications (Cambridge, England)·2026
Same author

Transient Electronic Polarizability of β-Carotene from Ultrafast Terahertz Stark Spectroscopy.

The journal of physical chemistry letters·2026
Same author

Large-Amplitude Charge Relocation in a Vibrationally Excited Molecular Crystal Probed by Femtosecond X-ray Diffraction.

The journal of physical chemistry letters·2025
Same author

Azide Anion Interactions with Imidazole and 1-Methylimidazole in Dimethyl Sulfoxide.

The journal of physical chemistry. B·2025
Same author

Solvated Electrons in Polar Liquids as ϵ-Near-Zero Materials Tunable in the Terahertz Frequency Range.

Physical review letters·2025
Same author

In vivo Study to Evaluate an Intelligent Algorithm for Time Efficient Detection of Malignant Melanoma Using Dermatofluoroscopy.

Skin pharmacology and physiology·2024

Related Experiment Video

Updated: May 12, 2026

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Note: an environmental cell for transient spectroscopy on solid samples in controlled atmospheres.

Jason R Dwyer1, Łukasz Szyc, Erik T J Nibbering

  • 1Department of Chemistry, 51 Lower College Rd., University of Rhode Island, Kingston, Rhode Island 02881, USA. jdwyer@chm.uri.edu

The Review of Scientific Instruments
|April 6, 2013
PubMed
Summary
This summary is machine-generated.

A novel sample cell was developed for time-resolved spectroscopy on solid samples under controlled atmospheres. Its design enables high-performance studies, particularly with femtosecond mid-infrared spectroscopy.

More Related Videos

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope
14:21

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope

Published on: July 24, 2021

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
06:06

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements

Published on: July 19, 2016

Related Experiment Videos

Last Updated: May 12, 2026

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope
14:21

Performing In Situ Closed-Cell Gas Reactions in the Transmission Electron Microscope

Published on: July 24, 2021

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements
06:06

A Simple Dewar/Cryostat for Thermally Equilibrating Samples at Known Temperatures for Accurate Cryogenic Luminescence Measurements

Published on: July 19, 2016

Area of Science:

  • Spectroscopy
  • Materials Science
  • Chemical Engineering

Background:

  • Time-resolved spectroscopy requires controlled environments for accurate solid-state analysis.
  • Existing methods may lack precise control over atmospheric composition or efficient sample handling.

Purpose of the Study:

  • To design and construct a specialized sample cell for time-resolved spectroscopy.
  • To enable studies on solid samples within precisely controlled vapor atmospheres.
  • To optimize the cell for femtosecond mid-infrared spectroscopy.

Main Methods:

  • Development of a passive sealing mechanism combined with chemical agents for vapor control.
  • Integration of ultrathin silicon nitride windows for enhanced optical access.
  • Implementation of a rapid and reproducible sample cell exchange system.

Main Results:

  • Successful design and construction of the specialized sample cell.
  • Demonstrated ability to maintain controlled vapor compositions.
  • Achieved performance characteristics suitable for advanced spectroscopic techniques.

Conclusions:

  • The developed sample cell provides a robust platform for time-resolved spectroscopy under controlled conditions.
  • The design facilitates high-fidelity measurements, especially in the mid-infrared region.
  • This advancement supports detailed investigations of solid-state dynamics.