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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
Twist2 functions as a tumor suppressor in murine osteosarcoma cells
Tomoki Ishikawa1, Takatsune Shimizu, Arisa Ueki
1Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, Tokyo, Japan.
Cancer Science
|April 6, 2013
Summary
Twist2 acts as a tumor suppressor in osteosarcoma, inhibiting cancer progression and metastasis. Its downregulation correlates with increased tumor formation, suggesting therapeutic potential for osteosarcoma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Epithelial-mesenchymal transition (EMT) drives cancer malignancy and metastasis.
- The role of EMT-transcription factors (EMT-TFs) in mesenchymal tumors like osteosarcoma is poorly understood.
Purpose of the Study:
- To investigate the role of Twist2, an EMT-TF, in osteosarcoma tumorigenesis.
- To determine if Twist2 functions as a tumor suppressor in osteosarcoma.
Main Methods:
- Analysis of Twist2 gene expression in human osteosarcoma.
- Forced expression and knockdown of Twist2 in murine osteosarcoma cell lines.
- In vitro cell growth assays and in vivo tumor formation studies.
- Assessment of fibulin-5 and MMP9 gene expression.
Main Results:
- Twist2 expression is downregulated in human osteosarcoma and inversely correlates with tumorigenic potential.
- Forced Twist2 expression suppressed osteosarcoma tumor formation in vivo.
- Twist2 knockdown enhanced tumor formation in vivo.
- Twist2 induced fibulin-5 expression and suppressed MMP9 expression and invasion.
Conclusions:
- Twist2 functions as a tumor suppressor in osteosarcoma.
- Twist2 inhibits osteosarcoma growth and metastasis by modulating the tumor microenvironment, including suppressing MMP9.
- Twist2 represents a potential therapeutic target for osteosarcoma.
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