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[Reference values of fasting serum levels of free amino acids in children]
N Liappis1, N Gobien, H Schlebusch
1Universitäts-Kinderklinik Bonn.
Klinische Padiatrie
|May 1, 1990
Insights
This study quantifies free amino acid levels in healthy children and adolescents. Results show age and sex influence these crucial nutrient concentrations.
Area of Science:
- Biochemistry
- Pediatric Nutrition
- Analytical Chemistry
Context:
- Establishing normative data for serum amino acid profiles is essential for pediatric health assessment.
- Previous studies may lack comprehensive age and sex stratification in pediatric populations.
- Accurate quantification requires validated analytical methods like ion exchange chromatography.
Purpose:
- To quantitatively determine serum concentrations of free amino acids in a large cohort of healthy infants, children, and adolescents.
- To present age-dependent and sex-dependent reference ranges for these biochemical markers.
- To provide a foundational dataset for future pediatric nutritional and metabolic research.
Summary:
- Serum samples from 352 healthy individuals (infants to adolescents) were analyzed for free amino acid concentrations using ion exchange column chromatography.
- Deproteinization was performed using 5% sulphosalicylic acid.
- Results, including 95% confidence ranges, are detailed across five age groups and stratified by sex.
Impact:
- Provides critical reference ranges for serum amino acids in pediatric populations.
- Aids in the diagnosis and management of metabolic and nutritional disorders in children.
- Serves as a benchmark for evaluating the impact of various factors on amino acid metabolism during growth and development.
Abstract:
Concentrations of free amino acids were determined quantitatively by ion exchange column chromatography in serum from the fasting blood of 352 (male = 177, female = 175) "healthy" infants, children and adolescents. Serum was deproteinised by 5% sulphosalicylic acid (1:1, v/v). The results -95% confidence range-are presented age-dependent (groups: up to 1 month, 1-24 months, 2-6 years, 6-10 years, 10-14 years) and sex-dependent in table 4 and 5.