Screening and evaluation of traditional Chinese medicine by microarray expression analysis
Guixiang Ren1, Xuemei Fan, Qionglin Liang
1School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Journal of Ethnopharmacology
|April 6, 2013
Summary
Salvia miltiorrhiza's lipophilic components (LCS) inhibit human umbilical vein endothelial cell proliferation and migration, suggesting potential for vascular anomaly hyperplasia treatment. Hydrophilic components (HCS) show opposite effects.
Area of Science:
- Pharmacology
- Molecular Biology
- Cell Biology
Background:
- Salvia miltiorrhiza is a traditional Chinese herb for cardiovascular conditions.
- Investigated effects of Salvia miltiorrhiza extracts on human umbilical vein endothelial cells (HUVECs).
- Explored molecular mechanisms using gene expression profiling.
Purpose of the Study:
- To evaluate the impact of Salvia miltiorrhiza hydrophilic (HCS) and lipophilic components (LCS) on HUVECs.
- To elucidate the molecular mechanisms underlying these effects via microarray analysis.
Main Methods:
- Assessed cell proliferation and migration to determine angiogenic effects.
- Employed microarray technology to profile gene expression in HUVECs treated with TES, HCS, and LCS.
- Utilized quantitative real-time PCR to validate microarray findings.
Main Results:
- LCS inhibited HUVEC proliferation and migration; HCS promoted them.
- TES did not affect HUVEC viability at 5 µg/mL.
- LCS significantly altered gene expression in HUVECs, with 511 genes changed, including increased IL-6 mRNA.
Conclusions:
- HCS and LCS from Salvia miltiorrhiza exert opposing effects on HUVECs.
- LCS demonstrates significant inhibitory action on HUVEC proliferation and migration.
- LCS may be applicable for vascular anomaly hyperplasia; involved pathways include ErbB, MAPK, p53, oxidative phosphorylation, and inflammatory response.
