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Updated: May 12, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

Enhanced lipid peroxidation and platelet activation as potential contributors to increased cardiovascular risk in the

Natale Vazzana1, Antonina Ganci, Angelo Baldassare Cefalù

  • 1Department of Internal Medicine and Center of Excellence on Aging, G. d'Annunzio University of Chieti, Chieti, Italy.

Journal of the American Heart Association
|April 6, 2013
PubMed

Insights

Low high-density lipoprotein (HDL) levels are linked to increased oxidative stress and platelet activation, contributing to cardiovascular risk. These findings highlight potential mechanisms connecting low HDL to atherothrombosis.

Area of Science:

  • Cardiovascular Medicine
  • Biochemistry
  • Lipid Metabolism

Background:

  • Low high-density lipoprotein (HDL) is a significant predictor of cardiovascular events, even in patients with controlled low-density lipoprotein (LDL).
  • HDL's protective roles in animal models include preventing LDL oxidation and reducing platelet activation.

Purpose of the Study:

  • To investigate the relationship between HDL levels and in vivo oxidative stress and platelet activation.
  • To explore the role of HDL in the mechanisms underlying atherothrombosis.

Main Methods:

  • Measured urinary markers of oxidative stress (8-iso-PGF2α) and platelet activation (11-dehydro-TXB2) in coronary heart disease (CHD) patients with varying HDL levels.
  • Assessed changes in these markers following intensive exercise and fenofibrate treatment in relevant subject groups.

Main Results:

  • Patients with low HDL (≤35 mg/dL) exhibited significantly higher levels of both 8-iso-PGF2α and 11-dehydro-TXB2 compared to those with higher HDL.
  • HDL levels were inversely correlated with both oxidative stress and platelet activation markers.
  • Oxidative stress (8-iso-PGF2α) and HDL level were independent predictors of platelet activation (11-dehydro-TXB2).

Conclusions:

  • A low HDL phenotype is associated with heightened lipid peroxidation and platelet activation.
  • These findings offer new insights into how low HDL contributes to increased cardiovascular risk.
Abstract

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