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Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Genomic DNA in human blastocoele fluid.
S Palini1, L Galluzzi, S De Stefani
1IVF Unit, Cervesi' Hospital Cattolica, 47841 Cattolica (Rn), Italy. simonepalini@yahoo.it
Reproductive Biomedicine Online
|April 6, 2013
Summary
Genomic DNA is present in blastocoele fluid from embryos undergoing vitrification. This finding allows for potential embryo sex determination and genetic testing, aiding in reproductive health decisions.
Area of Science:
- Reproductive Medicine
- Genetics
- Embryology
Background:
- Embryo cryopreservation via vitrification is standard in IVF.
- Embryo collapse during vitrification involves blastocoele fluid withdrawal.
- Blastocoele fluid metabolomics are studied for implantation efficiency prediction.
Purpose of the Study:
- To investigate the presence of embryonic genomic DNA in blastocoele fluid.
- To assess the feasibility of determining embryo sex from blastocoele fluid.
- To explore the potential for comprehensive genetic analysis of blastocoele fluid.
Main Methods:
- Real-time PCR was used to detect genomic DNA in blastocoele fluid.
- Amplification of Y-chromosome (TSPY1) and autosomal (TBC1D3) genes for sex determination.
- Whole-genome amplification and microarray comparative genomic hybridization were explored.
Main Results:
- Genomic DNA was detected in approximately 90% of blastocoele fluid samples.
- Embryo sex determination from blastocoele fluid was demonstrated.
- Whole-genome amplification and preliminary aneuploidy detection were feasible.
Conclusions:
- Blastocoele fluid is a source of embryonic DNA, enabling non-invasive sex determination.
- This technique could facilitate genetic screening for X-linked disorders.
- Further research is required to validate diagnostic accuracy for clinical application.
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