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In vivo MRS measurement of deoxymyoglobin in human forearms
Z Y Wang1, E A Noyszewski, J S Leigh
1Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104.
Abstract:
In vivo MRS measurement of deoxymyoglobin (deoxy-Mb) in human forearms was performed by observing the N-delta proton of F8 proximal histidine. The concentration of deoxy-Mb reflects the oxygen level in the muscle. In resting muscles, the deoxy-Mb level was below the detection sensitivity. When ischemia was introduced by arterial occlusion, the time-resolved NMR spectra (with 1 min resolution) was recorded to follow the change in the deoxy-Mb signal. The result shows that deoxy-Mb signal builds up and levels off within 6 min. This technique may be used to study bioenergetics of muscle during exercise and under pathological conditions.