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Updated: Jan 9, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Published on: August 11, 2021
Fluoxetine prevents acetylcholine-induced excitotoxicity blocking human endplate acetylcholine receptor
Cristina Deflorio1, Myriam Catalano, Sergio Fucile
1Fondazione Cenci Bolognetti, Dipartimento di Fisiologia e Farmacologia, Università Sapienza, Piazzale Aldo Moro 5, I-00185, Rome, Italy.
Introduction:
Fluoxetine is an open channel blocker of fetal muscle acetylcholine (ACh) receptor (AChR) and slow-channel mutant AChRs. It is used commonly to treat patients with slow-channel congenital myasthenic syndromes. Fluoxetine effects on adult wild-type endplate AChR are less characterized, although muscle AChR isoforms are differentially modulated by some drugs.
Methods:
Excitotoxicity assays and patch clamp recordings were performed in human embryonic kidney 293 (HEK) cells expressing wild-type or slow-channel mutant human AChRs.
Results:
Fluoxetine (2-10 μM) abolished ACh-induced death and decreased ACh-activated whole-cell currents in cells expressing all AChR types. In outside-out patches, fluoxetine rapidly curtailed ACh evoked unitary activity and macroscopic currents. The effect was increased if fluoxetine was applied before ACh.
Conclusions:
Fluoxetine is an open channel blocker, but it also affects AChR in the closed state. AChR blockade likely underlies the rescue of HEK cells from ACh-induced death.
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