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The 8-hydroxyguanine content of isolated mitochondria increases with lipid peroxidation
A M Hruszkewycz1, D S Bergtold
1Department of Pathology, George Washington University, Medical Center, Washington, DC 20037.
Mutation Research
|June 1, 1990
Abstract:
When lipid peroxidation was induced in isolated mitochondria there was a marked increase in the 8-hydroxyguanine content of the nucleic acids extracted from these mitochondria. The elevation of 8-hydroxyguanine levels was associated with an extensive alteration of normal electrophoretic mobility of mitochondrial DNA. However, suppression of lipid peroxidation with alpha-tocopherol proportionally attenuated 8-hydroxyguanine production and limited the electrophoretic mobility change of mitochondrial DNA.