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Related Concept Videos

Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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Related Experiment Video

Updated: May 12, 2026

Glycan Node Analysis: A Bottom-up Approach to Glycomics
11:36

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

Structural glycomic analyses at high sensitivity: a decade of progress.

William R Alley1, Milos V Novotny

  • 1Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)
|April 9, 2013
PubMed
Summary
This summary is machine-generated.

Advancements in analytical glycobiology, utilizing mass spectrometry and separation techniques, enable high-sensitivity structural characterization of complex carbohydrates. A multimethodological approach is crucial for understanding intricate glycomes in biological and medical research.

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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Related Experiment Videos

Last Updated: May 12, 2026

Glycan Node Analysis: A Bottom-up Approach to Glycomics
11:36

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Area of Science:

  • Glycomics and Analytical Glycobiology
  • Biomolecular Mass Spectrometry
  • Carbohydrate Chemistry

Background:

  • The urgent need for structural characterization and quantification of complex carbohydrates in biological and medical fields.
  • Significant progress in analytical glycobiology driven by technological advancements.

Purpose of the Study:

  • To highlight recent advances in glycomics.
  • To emphasize the necessity of a multimethodological approach for comprehensive glycome analysis.

Main Methods:

  • Biomolecular mass spectrometry and instrumentation.
  • Capillary and microchip separation techniques.
  • Microchemical manipulations of carbohydrate reactivity.

Main Results:

  • High-sensitivity analytical glycobiology achieved through technological integration.
  • Demonstration of the power of combining multiple analytical techniques.

Conclusions:

  • A multimethodological approach is essential for in-depth understanding of complex glycomes.
  • Continued advancements in analytical techniques are vital for progress in glycomics research.