Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.

G M Bonora1, C L Scremin, F P Colonna

  • 1Department of Organic Chemistry, University of Padova, Italy.

Nucleic Acids Research
|June 11, 1990
PubMed
Summary

This study introduces a fast and efficient method for synthesizing oligonucleotides using a soluble polymeric support. This approach yields significant amounts of short oligonucleotides with advantages over traditional solid-phase synthesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vibrational circular dichroism of polypeptides. 7. Film and solution studies of .beta.-sheet-forming homooligopeptides.

Journal of the American Chemical Society·2011
Same author

Synthesis by High-Efficiency Liquid-Phase (HELP) Method of Oligonucleotides Conjugated with High-Molecular Weight Polyethylene Glycols (PEGs).

Biological procedures online·2003
Same author

Triple, MPEG-conjugated, helix-forming oligonucleotides (TRIPEGXs): liquid-phase synthesis of natural and chimeric "all-purine" sequences linked to high molecular weight poly(ethylene glycols).

Bioconjugate chemistry·2001
Same author

New TFO conjugates containing a carminomycinone-derived chromophore.

Bioconjugate chemistry·2001
Same author

Nuclease resistance and RNase H sensitivity of oligonucleotides bridged by oligomethylenediol and oligoethylene glycol linkers.

Antisense & nucleic acid drug development·2001
Same author

PAcM-AN: poly (N-acryloylmorpholine)-conjugated antisense oligonucleotides.

Nucleosides, nucleotides & nucleic acids·2000

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Molecular Biology

Background:

  • Oligonucleotide synthesis is crucial for molecular biology and therapeutics.
  • Traditional solid-phase synthesis methods have limitations in scale and efficiency.
  • The phosphotriesters approach offers a viable route for oligonucleotide synthesis.

Purpose of the Study:

  • To develop a simple, rapid, and high-yielding method for oligonucleotide synthesis.
  • To explore the use of polyethylene glycol (PEG) as a soluble polymeric support.
  • To achieve large-scale synthesis of short oligonucleotides.

Main Methods:

  • Utilized the phosphotriesters approach for oligonucleotide synthesis.
  • Employed functionalized polyethylene glycol (PEG) as a soluble polymeric support.

Related Experiment Videos

  • Optimized reaction conditions for high yield and purity.

    • Main Results:

    • Achieved a simple, rapid, and high-yielding synthesis of oligonucleotides.
    • Demonstrated the advantages of using PEG as a soluble support, combining benefits of solid-phase synthesis with new features.
    • Successfully synthesized short oligonucleotides on a hundred milligram scale from grams of functionalized PEG.

    Conclusions:

    • The described method provides an efficient and scalable approach for oligonucleotide synthesis.
    • Polyethylene glycol (PEG) as a soluble support offers significant advantages for oligonucleotide synthesis.
    • This method is suitable for producing substantial quantities of short oligonucleotides.