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Comparison of enrichment and plating media for isolation of Yersinia
N A Cox1, J S Bailey, F Del Corral
1USDA/ARS-Richard B. Russell Agricultural Research Center, Athens, Georgia 30613.
Abstract:
Yersinia enterocolitica (Serotypes 0:3 or 0:8), Yersinia frederiksenii, Yersinia kristensenii, or Yersinia intermedia along with 10(8) cells of each of three extraneous organisms (Escherichia coli, Enterobacter aerogenes, Pseudomonas fragi), all commonly found on market poultry, were inoculated into five enrichment media followed by streaking onto 11 plating media to determine the most-efficacious combinations for future surveys or assessment studies. For Yersinia enterocolitica (0:8), infrequent recoveries were made using yeast extract-rosebengal-bile oxalate sorbose broth and phosphate-buffered saline (4 C) followed by plating onto pectin, DNase-Tween 80 (polyoxyethylene sorbitan monooleate)-sorbitol, MacConkey-Tween 80, or cefsulodin-irgasan-novobiocin (CIN) agars. With Y. enterocolitica (0:3), recoveries were most frequently made using phosphate-buffered saline, sorbitol bile (incubated for 17 days) and yeast extract-rosebengal-bile oxalate sorbose broth followed by plating onto pectin, CIN, bismuth sulfite (Difco Laboratories, Detroit, MI), or modified Rimler-Shotts agar. For Y. frederiksenii, Y. kristensenii, and Y. intermedia, incubation in sorbitol bile for 17 days or in yeast extract-rosebengal-bile oxalate sorbose broth, followed by plating onto CIN, pectin, DNase-Tween/80-sorbitol, cellobiose-arginine-lysine agar, or MacConkey-Tween 80 agar yielded the most-frequent recoveries. Overall, the CIN and pectin agars performed best for the recovery of the Yersinia bacterium; the modified selenite broth and the bismuth-sulfite plating agars were unsatisfactory in the present study.
Insights
This study evaluated enrichment and plating media for Yersinia recovery from poultry. Cefsulodin-irgasan-novobiocin (CIN) and pectin agars were most effective for Yersinia detection.
Area of Science:
- Microbiology
- Food Safety
- Veterinary Science
Background:
- Yersinia species are significant foodborne pathogens, frequently isolated from market poultry.
- Accurate detection of Yersinia in poultry is crucial for public health and food safety assessments.
- Optimizing detection methods is essential for reliable surveillance and control strategies.
Purpose of the Study:
- To identify the most effective combinations of enrichment and plating media for recovering Yersinia species from poultry.
- To compare the efficacy of various media for detecting Yersinia enterocolitica (serotypes 0:3 and 0:8), Yersinia frederiksenii, Yersinia kristensenii, and Yersinia intermedia.
Main Methods:
- Five enrichment media and eleven plating media were tested against Yersinia species and common poultry contaminants.
- Inoculated cultures were subjected to specific incubation conditions before plating.
- Recovery rates of Yersinia were assessed on different agar types.
Main Results:
- Cefsulodin-irgasan-novobiocin (CIN) agar and pectin agar demonstrated the highest recovery rates for Yersinia species overall.
- Yersinia enterocolitica serotype 0:3 showed optimal recovery with specific enrichment broths followed by CIN or pectin agar.
- Modified selenite broth and bismuth sulfite agar were found to be unsatisfactory for Yersinia detection in this study.
Conclusions:
- CIN agar and pectin agar are recommended for future surveys and assessment studies of Yersinia in poultry.
- Specific media combinations can enhance the detection of different Yersinia serotypes.
- The study provides valuable insights for improving Yersinia detection protocols in food safety.