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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features.

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

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Published on: March 30, 2015

New Developments in Quantitative Real-time Polymerase Chain Reaction Technology.

Vija yJ Gadkar1, Martin Filion

  • 1Department of Biology, Universite de Moncton, 18 Antonine-Maillet, Moncton, NB E1A 3E9 Canada.

Current Issues in Molecular Biology
|April 9, 2013
PubMed
Summary

Real-time quantitative PCR (RT-qPCR) technology has advanced significantly, becoming more sensitive, faster, and affordable. Recent developments in nucleic acid amplification and RT-qPCR components enhance its performance for molecular biology research.

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Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Real-time quantitative PCR (RT-qPCR) is a cornerstone technology in molecular biology.
  • Its fundamental principles remain consistent, but continuous improvements have boosted its performance.

Purpose of the Study:

  • To summarize recent advancements in RT-qPCR technology.
  • To review developments in nucleic acid amplification techniques.

Main Methods:

  • Review of recent literature and technological innovations in RT-qPCR.
  • Analysis of modifications in enzymatic reaction components and detection systems.

Main Results:

  • RT-qPCR technology has become more sensitive, faster, and cost-effective.
  • Improvements span across reaction cocktails (enzymes, buffers, probes) and detection instrumentation/software.

Conclusions:

  • Ongoing developments continue to enhance the versatility and efficiency of RT-qPCR.
  • These advancements solidify RT-qPCR's role in molecular biology detection.