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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
07:06

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Published on: April 3, 2017

Foetal stem cell derivation & characterization for osteogenic lineage.

A Mangala Gowri1, G Kavitha, M Rajasundari

  • 1Department of Animal Biotechnology, Madras Veterinary College, Chennai, India. gowrivalavan@hotmail.com

The Indian Journal of Medical Research
|April 9, 2013
PubMed
Summary

Researchers successfully isolated ovine fetal bone marrow mesenchymal stem cells (MSCs) and confirmed their osteogenic differentiation potential. This ovine model offers a valuable resource for regenerative medicine and tissue engineering applications.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) from fetal tissues are multipotent progenitors for regenerative medicine.
  • Ovine fetal mesenchymal stem cells (MSCs) can serve as a preclinical model for human applications.

Purpose of the Study:

  • To isolate and characterize ovine fetal bone marrow-derived MSCs.
  • To analyze their differentiation potential into the osteogenic lineage.
  • To establish an ovine animal model for predicting human regenerative therapies.

Main Methods:

  • Isolation and culture of ovine fetal bone marrow cells.
  • Characterization via cytochemical, immunophenotyping, biochemical, and molecular analyses.
  • Osteogenic differentiation induction and analysis of gene and protein expression.

Main Results:

  • Established a protocol for isolating ovine fetal MSCs with spindle morphology.
  • Confirmed MSCs express specific surface markers (CD44, CD54, integrinβ1) and intracellular proteins (collagen type I/III, fibronectin).
  • Demonstrated osteogenic differentiation via alkaline phosphatase activity, mineral deposition, and gene expression (collagen type I, MMP13).

Conclusions:

  • A simple protocol for ovine fetal MSC isolation and culture was developed.
  • Defined criteria for MSC identification including adherence, surface markers, and osteogenic differentiation.
  • Ovine fetal MSCs represent a promising source for stem cell applications in regenerative medicine.