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Isolation of campesteryl ferulate and epi-campesteryl ferulate, two components of γ-oryzanol from rice bran
Yuhua Bao1, Emiko Yanase, Shin-ichi Nakatsuka
1The United Graduate School of Agricultural Science, Gifu University.
Bioscience, Biotechnology, and Biochemistry
|April 9, 2013
Abstract:
Campesteryl ferulate (3a, 24R/α) and epi-campesteryl ferulate (3b, 24S/β), components of rice bran γ-oryzanol, were isolated by the preparative recycle HPLC system using a combination of ODS silica and cholester packed columns at over 99% purity. Their purities and structures of 3a and 3b thus obtained were confirmed by HPLC analysis and physical data (1H- and 13C-NMR, MS spectra, and X-ray crystallography).

