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Updated: May 12, 2026
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Published on: September 28, 2016
Ion channels
1Department of Infection, Immunity and Inflammation, Institute for Lung Health, University of Leicester, , Leicester, UK.
Abstract:
Effective therapies for the 10% of patients with 'severe' asthma remain elusive, while other pulmonary diseases such as idiopathic pulmonary fibrosis are currently untreatable. Many cellular processes rely heavily on signals delivered by changes in the intracellular-free Ca(2+) concentration, in many cases relying on Ca(2+) influx from the extracellular fluid through specific ion channels. This Ca(2+) influx is, to some extent, dependent on the plasma membrane potential, which is controlled by the flow of other ions such as K(+) and Cl(-) through their own channels. Irrespective of the point from where pathophysiological cell function is sustained, all mechanisms are predicted to rely heavily on the activity of the final effector ion channels required for pathological cell function. Ion channels are therefore highly attractive targets for the treatment of many diverse diseases, including those affecting the lung.
Insights
Ion channels controlling calcium (Ca2+) influx are crucial for cellular processes and disease development. Targeting these ion channels offers a promising therapeutic strategy for severe asthma and other lung diseases.
Area of Science:
- Biochemistry
- Cell Biology
- Pulmonary Medicine
Background:
- Severe asthma and idiopathic pulmonary fibrosis lack effective treatments.
- Cellular functions depend on intracellular calcium (Ca2+) concentration.
- Ca2+ influx is regulated by plasma membrane potential and ion channels.
Purpose of the Study:
- To highlight the critical role of ion channels in cellular signaling.
- To propose ion channels as therapeutic targets for lung diseases.
Main Methods:
- Review of cellular mechanisms involving ion transport.
- Analysis of the role of Ca2+ and other ion channels in disease pathophysiology.
Main Results:
- Ion channel activity is fundamental to pathological cellular functions.
- Ca2+ influx, regulated by membrane potential and ion channels, is key.
Conclusions:
- Ion channels are essential effectors in various disease mechanisms.
- Targeting ion channels presents a viable therapeutic avenue for diverse diseases, including pulmonary conditions.
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