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Related Concept Videos

Biofuels01:25

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

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Related Experiment Video

Updated: May 12, 2026

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases
08:41

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases

Published on: December 19, 2019

Microplates as a microreactor platform for microalgae research.

Adriana Pacheco1, Ileana Hernández-Mireles, Cecilia García-Martínez

  • 1Escuela de Biotecnología y Alimentos, Tecnológico de Monterrey, Campus Monterrey, Eugenio Garza Sada 2501, 64849, Monterrey, NL, México. adrianap@itesm.mx

Biotechnology Progress
|April 9, 2013
PubMed
Summary

96-well microplates are feasible for microalgae research, despite growth variations between well positions. Excluding border wells and using randomization ensures statistically robust results for high-throughput screening.

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Last Updated: May 12, 2026

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases
08:41

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases

Published on: December 19, 2019

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

High-Throughput Metabolic Profiling for Model Refinements of Microalgae
11:07

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

Area of Science:

  • Biotechnology
  • Phycology
  • Microalgae Cultivation

Background:

  • Commercial high-throughput screening platforms for microalgae are lacking.
  • 96-well microplates offer a potential microreactor system for microalgae research.
  • Investigating well-to-well variability is crucial for microplate-based assays.

Purpose of the Study:

  • To assess the feasibility of 96-well microplates for microalgae research.
  • To evaluate well equivalence as microreactors under controlled high CO2 conditions.
  • To determine the statistical robustness of microplate assays for microalgae.

Main Methods:

  • Assessed growth rates of Scenedesmus sp. UTEX1589 and an environmental isolate in 96-well plates.
  • Analyzed growth rate gradients and coefficients of variation (CV).
  • Conducted a demonstrative experiment on media dilution effects across six microalgae species using a randomized design.

Main Results:

  • Microalgae growth rates were higher in border wells compared to internal wells.
  • Growth rate gradients were observed for Scenedesmus sp.
  • High precision was achieved (CV 6.8-7.8%), and media dilution up to 50% (v/v) showed no detrimental effect (CV 9.4-12.9%).

Conclusions:

  • 96-well microplates are a practical and statistically robust platform for microalgae research.
  • Excluding border wells and employing a randomized design are recommended for reliable results.
  • This approach supports efficient microalgae screening and experimentation.