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Related Concept Videos

Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
Acute Pyelonephritis I: Introduction01:27

Acute Pyelonephritis I: Introduction

Pyelonephritis is a bacterial infection that primarily affects the renal parenchyma and collecting system, including the renal pelvis, tubules, and interstitial tissue of one or both kidneys. It can be classified as either acute—a sudden, severe infection—or chronic, which refers to long-term or recurrent kidney infections.The primary cause of acute pyelonephritis (APN) is bacterial infection, with Escherichia coli accounting for approximately 70-80% of cases. Other bacteria, such as Proteus,...

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Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis
06:09

Intrarenal Injection of Escherichia coli in a Rat Model of Pyelonephritis

Published on: July 18, 2017

Mouse model for pyelonephritis.

André P Tittel1, Christoph Heuser, Christian Kurts

  • 1Institutes of Molecular Medicine and Experimental Immunology, Rheinische Friedrich-Wilhelms University, Bonn, Germany.

Current Protocols in Immunology
|April 9, 2013
PubMed
Summary

This study details a method for inducing pyelonephritis in female mice using uropathogenic Escherichia coli (UPEC). It provides protocols for bacterial culture, infection, and quantifying bacterial load in kidneys via colony-forming units (CFU).

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Area of Science:

  • Microbiology
  • Immunology
  • Animal Models

Background:

  • Pyelonephritis is a significant kidney infection often caused by uropathogenic Escherichia coli (UPEC).
  • Developing reliable animal models is crucial for studying pyelonephritis pathogenesis and testing therapeutics.
  • Standardized protocols are needed for reproducible induction and assessment of UPEC-induced kidney infections in mice.

Purpose of the Study:

  • To describe a straightforward protocol for inducing pyelonephritis in female mice using UPEC.
  • To provide detailed methods for culturing UPEC and preparing bacterial stocks for infection.
  • To establish a quantitative assay for measuring bacterial load in murine kidneys.

Main Methods:

  • Uropathogenic Escherichia coli (UPEC) strains were cultured and prepared into working stocks.
  • Female mice were inoculated to establish experimental pyelonephritis.
  • Bacterial load in kidneys was quantified by determining colony-forming units (CFU) from homogenates or single-cell suspensions.
  • Kidney cells were isolated for downstream analyses like flow cytometry.

Main Results:

  • The protocol successfully induced pyelonephritis in female mice.
  • Quantification of bacterial load using CFU was feasible from both kidney homogenates and single-cell suspensions.
  • Isolated kidney cells were suitable for various downstream assays.

Conclusions:

  • This unit provides a reproducible method for inducing and assessing UPEC-induced pyelonephritis in a murine model.
  • The described protocols facilitate the study of host-pathogen interactions and immune responses in kidney infections.
  • The methodology supports further investigations into pyelonephritis using various cellular and molecular assays.