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Updated: May 12, 2026
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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds
Roman J Lichtenecker1, Nicolas Coudevylle, Robert Konrat
1Institute of Organic Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria. roman.lichtenecker@univie.ac.at
Chembiochem : a European Journal of Chemical Biology
|April 9, 2013
Abstract:
You can have one without the other: A new metabolic precursor compound can selectively introduce (13)C and (2)H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.
