Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026
Same author

Structural Insights into AgBi(SC<sub>12</sub>H<sub>25</sub>)<sub>4</sub> Mixed-Metal <i>n</i>-Alkanethiolate: Heterometallic Thiolate Bridging and Metallophilic Interaction-Directed Self-Assembly.

Inorganic chemistry·2026
Same author

Moiré-pattern-assisted thermoelectric enhancement in tungsten diselenide bilayer.

Physical chemistry chemical physics : PCCP·2026
Same author

Supramolecular Assembly of a Heavy-Atom-Free Highly Efficient Self-Deliverable Photosensitizer for Activable Photodynamic Therapy.

Angewandte Chemie (International ed. in English)·2026
Same author

Molecular dynamics analyses of CLDN15 pore size and charge selectivity.

The Journal of general physiology·2026
Same author

Prediction of Negative Thermal Expansion in 2D-Materials by Multistep Machine Learning Using Structural Descriptors.

Chemistry, an Asian journal·2026

Related Experiment Video

Updated: May 12, 2026

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Molecular switching behavior in isosteric DNA base pairs.

A K Jissy1, Sukanya Konar, Ayan Datta

  • 1School of Chemistry, Indian Institute of Science Education and Research Thiruvananthapuram, CET Campus, Thiruvananthapuram 695016, Kerala, India.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|April 9, 2013
PubMed
Summary

Adenine-thymine (A-T) and adenine-difluorotoluene (A-F) base pairs were studied. The A-F pair is favored in low dielectric solvents and stabilizes in acidic conditions, enabling pH-controlled molecular switches.

More Related Videos

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Related Experiment Videos

Last Updated: May 12, 2026

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Area of Science:

  • Computational chemistry
  • Molecular biophysics
  • Biochemistry

Background:

  • DNA base pairing is fundamental to genetic information storage and replication.
  • Modified nucleobases offer opportunities to engineer novel DNA functions.
  • Understanding base pair stability in different environments is crucial for DNA nanotechnology.

Purpose of the Study:

  • To investigate the structural and proton-coupled behavior of adenine-thymine (A-T) and adenine-difluorotoluene (A-F) base pairs.
  • To analyze the stability of these base pairs in various solvents and under different pH conditions.
  • To explore the potential of modified base pairs in developing pH-based molecular switches.

Main Methods:

  • Dispersion-corrected density functional theory (DFT) calculations were employed.
  • The study analyzed base pair structures and stability in solvents with varying dielectric constants.
  • Protonation effects on base pair stabilization and conformational changes were investigated.

Main Results:

  • Adenine-difluorotoluene (A-F) base pairing is favored in solvents with low dielectric constants.
  • Protonation significantly stabilizes the A-F base pair, particularly in acidic media.
  • The study identified conformational changes induced by protonation in both natural and modified base pairs.

Conclusions:

  • The adenine-difluorotoluene (A-F) base pair exhibits unique stability characteristics in different solvent environments.
  • Protonation-induced stabilization of the A-F pair suggests its utility in pH-responsive DNA systems.
  • Modified base pairs like A-F can be integrated into DNA to create functional pH-controlled molecular switches.