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Updated: May 12, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Interleukin polymorphisms and differential methylation status in gastric cancer: an association with Helicobacter
Débora Menezes da Costa1, Eduardo Henrique Cunha Neves-Filho, Markênia Kélia Santos Alves
1Universidade Federal do Ceará, Department of Pathology & Forensic Medicine, Rua Alexandre Baraúna, 949, Porangabussu, CEP 60183-630, Fortaleza, Brazil. debora_mcosta@hotmail.com
Aim:
Interleukin polymorphisms and Helicobacter pylori infection are believed to play critical roles in DNA methylation, a process frequently associated with carcinogenesis. The aim of this study was to determine the associations between interleukin polymorphisms and methylation status of three genes related to gastric cancer. Furthermore, the influence of the H. pylori strains was evaluated.
Materials & Methods:
75 gastric tumor samples had the DNA extracted for interleukin polymorphisms genotyping by PCR-RFLP, promoter methylation by MS-PCR and detection and subtyping of H. pylori by PCR.
Results:
In the cardia tumors, methylation in the COX-2 promoter was associated with IL1RN*2 (p = 0.015), and the associated genotypes IL1B511T + IL1RN*2 seem to be important in the methylation of COX-2 (p = 0.013), especially in the presence of cagA(+) (p = 0.026) and vacAs1 (p = 0.025) H. pylori strains. The associated genotypes IL6 CC+TNF GG seem to be involved in the unmethylation of CDKN2A (p = 0.046), along with H. pylori cagA(+) infection.
Conclusion:
DNA methylation in gastric cancer seems to be influenced by the presence of interleukin polymorphisms and by the H. pylori cagA/vacAs1m1 strains.
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