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Updated: May 12, 2026

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
Published on: April 30, 2020
A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis
Balaji Venkataraman1, Nidhi Gupta, Amita Gupta
1Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Abstract:
We describe a robust method for the isolation of pure, intact RNA suitable for transcriptome studies from mycobacteria with consistent yields of 1 μg to 3 μg total RNA per 10(7) cells. The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA.
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