A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs
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Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
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Tomoshige Matsumoto1, Yuriko Koshii, Kazu Sakane
1Osaka Prefectural Medical Center for Respiratory and Allergic Diseases, Japan. tom_matsumoto@sutv.zaq.ne.jp
Journal of Microbiological Methods
|April 10, 2013
Abstract:
In this study, we present a new approach to variable number tandem repeats (VNTR) analysis using the QIAxcel capillary electrophoresis system and a software-integrated peak calling function. Allelic ladders representing 15 mycobacterial interspersed repetitive units (MIRU)-VNTR loci were used to define peak calling tables thereby enabling high precision Mycobacterium tuberculosis strain identification.

