Related Experiment Video
Updated: May 12, 2026

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
Published on: December 16, 2016
An oligopeptide transporter gene family in Phanerochaete chrysosporium
Quanju Xiang1, Zhongshan Wang, Yizheng Zhang
1College of Life Sciences, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Sichuan University, Chengdu 610064, PR China.
Abstract:
Oligopeptide transporters are believed to translocate their substrates across the membrane from the extracellular environment into cell. In the present study, sixteen oligopeptide transporter (OPT) orthologs (PcOPT1 to PcOPT16) were identified in Phanerochaete chrysosporium strain BKMF1767. They were predicted to encode integral membrane proteins with 13 to 16 transmembrane domains. The cDNA and genomic sequences of these genes were confirmed by clone sequencing and Transcriptome Sequencing. Based on sequence similarities, the PcOPT genes can be grouped into three different subfamilies. Comprehensive analysis revealed correlations between gene groupings and gene structures. The PcOPT genes showed differential expression patterns at different metabolic stages or in various stress conditions, suggesting that they may play different functional roles. PcOPT14 was able to mediate the uptake of tetrapeptide KLGL when heterologously expressed in Saccharomyces cerevisiae. This study provides some basic genomic information for OPT genes in P. chrysosporium and will be useful for elucidating their roles in growth, stress response and survival of this organism. Moreover, the results can help us to better understand the complexity of metabolism of P. chrysosporium.
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Thylakoids
Protein Translocation Machinery on the ER Membrane
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
ABC Transporters: Exporter

