Specific expression pattern of a novel Otx2 splicing variant during neural differentiation
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Updated: May 12, 2026

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
Published on: February 2, 2016
Zhongliang Liu1, Liankai Chi, Yujiang Fang
1Shanghai Tenth People's Hospital and Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China.
Abstract:
We cloned a new splicing variant of Otx2 gene, Otx2c. Otx2c lacks entire exon 4, most of the region encoding the homeodomain. More importantly, Otx2c harbors an early premature stop codon and bioinformatics analysis prefers it to be a non-protein coding RNA. In addition, this splicing variant is not simply a noise during mRNA processing, since it is mainly expressed in undifferentiated human embryonic stem cells but gradually decreased during differentiation. Therefore, we report here that a single pre-mRNA can generate both coding and non-coding RNAs through alternative splicing and this splicing activity is tightly regulated in different cell contexts.
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