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Updated: May 12, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
Published on: January 7, 2019
Pentamerisation of a scFv directed against TRAIL receptor 2 increases its antitumour efficacy
1CAS Key Laboratory of Pathogenic Microbiology and Immunology-CASPMI, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PRC.
Abstract:
Single-chain variable fragments (scFvs) have been considered as promising therapeutic drugs. However, their low affinity and rapid clearance from the blood have limited their wider application clinically. In this study, we aimed to improve scFv antibodies by multimerising a scFv against the death receptor 5 (DR5, TNFR10B) through fusion with a coiled-coil domain of human cartilage oligomeric matrix protein 48. By forming a pentamer, nick-named a combody, the avidity of the scFv increased 10⁴-fold and combody was more effective than its monomeric counterpart for antitumour activity in both in vitro and in vivo experiments.
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