Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metasurface integrated optics for fluorescence imaging endoscopy.

Optics express·2026
Same author

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy.

Biomedical optics express·2024
Same author

Metaoptics for aberration correction in microendoscopy.

Optics express·2024
Same author

Transform-based phase retrieval techniques from a single off-axis interferogram.

Applied optics·2021
Same author

All-digital Stokes polarimetry with a digital micromirror device.

Optics letters·2020
Same author

MetaOptics: opensource software for designing metasurface optical element GDSII layouts.

Optics express·2020

Related Experiment Video

Updated: May 12, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Improvements in speckle tracking algorithms for vibrational analysis using optical coherence tomography.

Prashanth Raghavendra Prasad1, Shanti Bhattacharya

  • 1Indian Institute of Technology Madras, Department of Electrical Engineering, Chennai, India.

Journal of Biomedical Optics
|April 10, 2013
PubMed
Summary

Improved speckle tracking algorithms enhance optical coherence tomography for subsurface vibration analysis. This advancement allows for precise depth-resolved measurements, paving the way for better optical coherence elastography.

More Related Videos

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
19:16

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Related Experiment Videos

Last Updated: May 12, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
19:16

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

Area of Science:

  • Biomedical Optics
  • Vibrational Analysis
  • Medical Imaging

Background:

  • Optical coherence tomography (OCT) is a valuable tool for subsurface imaging.
  • Analyzing subsurface vibrations requires precise and sensitive measurement techniques.
  • Existing speckle tracking methods may have limitations in resolution and accuracy.

Purpose of the Study:

  • To improve speckle tracking algorithms for analyzing subsurface vibrations using OCT.
  • To achieve depth-resolved measurements of displacement, velocity, and vibration frequencies.
  • To enhance the capabilities of optical coherence elastography.

Main Methods:

  • Developed an improved speckle tracking technique incorporating axial deconvolution and linear interpolation.
  • Applied cross-correlation-based speckle tracking to OCT depth scans.
  • Validated the method on M-mode images of a test sample and human wrist pulses.

Main Results:

  • Successfully tracked speckle pattern displacements and features as small as 0.5 pixels.
  • Achieved depth-resolved measurements of vibration displacement, velocity, and frequencies.
  • Demonstrated the potential for enhanced performance in optical coherence elastography.

Conclusions:

  • The improved speckle tracking algorithm significantly enhances subsurface vibration analysis with OCT.
  • The technique provides high-resolution, depth-resolved vibrational measurements.
  • Extending this method to two dimensions holds promise for advancing optical coherence elastography.