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Updated: May 12, 2026
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Identification of low abundance proteins in colorectal cancer tissues
Shi Rou Lim1, Boon-Hui Gooi, Lay Harn Gam
1School of Pharmaceutical Sciences, University Sains Malaysia, and Surgery Department, Penang General Hospital, Penang, Malaysia.
Background:
Detection of low abundance proteins always possesses challenges even with the currently available proteomics technologies.
Objective:
We aimed to evaluate the low abundance differentially expressed proteins in colorectal cancerous tissues as compared to colorectal normal tissues.
Methodology:
Protein separation was carried out by using the coupling methods of hydroxyapatite chromatography and SDS-PAGE followed by mass spectrometry analysis.
Results:
Five of the membrane associated low abundance proteins, namely uncharacterized protein C5orf4, coiled-coil domain containing protein 152, DnaJ homolog subfamily C member 22, tumor suppressor candidate 3 and leucine-rich repeat transmembrane neuronal protein 4 that previously only reported at the genome level were identified.
Conclusions:
The proteins were isolated from the cancerous tissues as either up-regulated or down-regulated proteins as compared to normal tissues and therefore may play important roles in cancer development.
