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Updated: May 12, 2026
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Published on: October 15, 2021
[Identification of a novel HLA allele HLA-B*55:35]
Yong-hong Song1, Yong-xin Mao, Kui Dong
1China Marrow Donor Program HLA Typing Laboratory, Blood Center of Shandong Province, Jinan, P.R. China.
Objective:
To identify a novel human leukocyte antigen (HLA) B allele and explore its family heritage.
Methods:
A novel HLA allele was suspected upon routine HLA typing using a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) assay. The sequence was confirmed with DNA sequencing and compared with its closest matching allele, B*55:02. The family was also investigated.
Results:
An unusual reaction pattern was detected during routine HLA typing. The sequence was confirmed to be a novel HLA-B allele, which differed from the closest matching allele, B*55:02 in 7 nt positions in exon 2. Among the 7 mutations from 6 codons, there were two amino acids changes including 69Glu→Met and 70Glu→Ala.
Conclusion:
A novel HLA-B allele has been identified and officially named as B*55:35 by the WHO Nomenclature Committee for Factors of the HLA System (GenBank accession number FJ898284).
