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Updated: May 12, 2026
Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
Published on: September 1, 2009
[Analysis of 89 amniotic samples using fluorescent in situ hybridization]
Ping Wei1, Yun-xing Li, Lan Zeng
1Sichuan Provincial Hospital for Women and Children and Sichuan Prenatal Diagnosis Center, Chengdu, P.R. China.
Fluorescent in situ hybridization (FISH) accurately detects chromosome aneuploidies in prenatal samples. This rapid FISH method offers a reliable alternative for prenatal diagnosis, even with failed amniotic fluid cell cultures.
Area of Science:
- Cytogenetics
- Molecular Biology
- Prenatal Diagnostics
Context:
- Amniotic fluid cell culture is standard for prenatal diagnosis.
- Culture failures can delay or prevent diagnosis.
- Detecting common chromosome aneuploidies is crucial for fetal health assessment.
Purpose:
- To evaluate the efficacy of fluorescent in situ hybridization (FISH) for identifying common chromosome aneuploidies in interphase nuclei of amniotic fluid cells.
Summary:
- FISH analysis was performed on 89 amniotic fluid samples (82 uncultured, 7 cultured).
- Results from FISH were compared with standard cytogenetic analysis.
- FISH successfully identified trisomy 21, 47,XYY, and 69,XXX, aligning with karyotype findings.
Impact:
- FISH provides a rapid and accurate method for prenatal diagnosis.
- This technique serves as a valuable alternative when amniotic fluid cell cultures fail.
- FISH enhances the reliability and efficiency of prenatal screening for chromosomal abnormalities.
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