Related Experiment Video
Updated: May 12, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Enantioseparation of chiral aromatic acids by multiple dual mode counter-current chromatography using
Shengqiang Tong1, Ye Zheng, Jizhong Yan
1College of Pharmaceutical Science, Zhengjiang University of Technology, Hangzhou, China. sqtong@zjut.edu.cn
Abstract:
This work concentrates on extending the utilization of multiple dual mode (MDM) counter-current chromatography in chiral separations. Two aromatic acids, 2-(6-methoxy-2-naphthyl)propionic acid (NAP) and 2-phenylpropionic acid (2-PPA), were enantioseparated by MDM counter-current chromatography using hydroxypropyl-β-cyclodextrin (HP-β-CD) as chiral selector. The two-phase solvent systems consisting of n-hexane/ethyl acetate 0.1 mol/L phosphate buffer pH 2.67 containing 0.1 mol/L HP-β-CD (7.5:2.5:10 for NAP and 7:3:10 for 2-PPA, v/v/v) were used. Conventional MDM and modified MDM were compared according to peak resolution under current separation mechanism. The influence of elution time after the first-phase inversion and number of cycles for MDM were investigated. Peak resolution of NAP and 2-PPA increased from 0.62 to 1.05 and 0.72 to 0.84, respectively, using optimized MDM conditions. Being an alternative elution method for counter-current chromatography, MDM elution greatly improved peak resolution in chiral separations.
Related Concept Videos
Ion-Exchange Chromatography
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Regioselectivity and Stereochemistry of Acid-Catalyzed Hydration
Prochirality

