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Published on: August 1, 2018
Protein chemical synthesis by serine and threonine ligation
Yinfeng Zhang1, Ci Xu, Hiu Yung Lam
1Department of Chemistry, The University of Hong Kong, Hong Kong, People's Republic of China.
Abstract:
An efficient method has been developed for the salicylaldehyde ester-mediated ligation of unprotected peptides at serine (Ser) or threonine (Thr) residues. The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (∼11 kDa). The requisite peptide salicylaldehyde ester precursor is prepared in an epimerization-free manner via Fmoc-solid-phase peptide synthesis.
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