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Published on: June 15, 2017
Immunohistochemical study of the digestive tract of Oligosarcus hepsetus
Danielle A Vieira-Lopes1, Nadja L Pinheiro, Armando Sales
1Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil. dmjvieira@hotmail.com
Aim:
To describe the histology of the digestive tract and to investigate the occurrence of endocrine cells in Oligosarcus hepsetus (O. hepsetus).
Methods:
The digestive tract (DT) of O. hepsetus was divided into esophagus, two stomach regions (glandular and non-glandular) and two intestinal regions (anterior and posterior). These specimens were processed by routine histological techniques and stained with hematoxylin-eosin, Gomori's trichrome, periodic acid Schiff (PAS) and Alcian blue (AB). An immunohistochemical method using avidin-biotin-peroxidase was employed.
Results:
The esophagus is lined with a non-keratinized stratified squamous epithelium that is reactive to PAS and AB. The stomach has a mucosa lined with a simple columnar epithelium with mucus-secreting cells that are reactive only to PAS. The intestine has a simple columnar epithelium with a brush border and goblet cells that are reactive to PAS and AB. Somatostatin, serotonin and cholecystokinin immunoreactive cells were identified throughout the DT.
Conclusion:
This study revealed adaptations for the species' diet and showed that the distribution and relative frequency of immunoreactive cells are similar to those of other fish.
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