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A blotting method for monitoring the formation of chemically induced DNA-protein complexes
M D Cohen1, C A Miller, L S Xu
1Department of Environmental Medicine, New York University Medical Center, New York 10016.
Analytical Biochemistry
|April 1, 1990
Abstract:
The formation and identification of DNA-protein crosslinks are usually detected by filter binding assays such as alkaline elution. We describe a modified blotting method to selectively identify DNA-protein complexes (DPCs) formed in vitro by either Cr3+ ion or formaldehyde. This protocol allows DPC formation in vitro to be assayed with various chemical agents, requires minimal usage of radioactivity, and is performed in a shorter time frame than that commonly used to resolve DPCs from free proteins and unbound DNA.