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Effect of bacteriophage lysin on lysogens
Balaji Subramanyam1, Vanaja Kumar
1Tuberculosis Research Centre, Chennai, India.
Objective:
To study the effect of phage lysin on the growth of lysogens.
Methods:
Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18-24 hours. The growth of normal flora observed after day 1 was considered as lysogens.
Results:
Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysin alone, lysogen formation was avoided and normal flora was controlled.
Conclusions:
Lysin may have no effect on the growth of lysogens.
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