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Related Experiment Video

Updated: May 12, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

A quantitative high-throughput endothelial cell migration assay.

Vladimir Mastyugin1, Elizabeth McWhinnie, Mark Labow

  • 1Novartis Institutes for Biomedical Research, Cambridge, MA, USA.

Drug Discovery Today
|April 11, 2013
PubMed
Summary

A new cell migration assay using FluoroBlok Boyden chambers and Cellomics HCS ArrayScan offers a 10-fold increase in sensitivity. This method accurately measures endothelial cell migration and screens compounds for growth factor-mediated effects.

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Pharmacology

Background:

  • Traditional cell migration assays often lack sensitivity.
  • Measuring total well fluorescence is a common but limited approach.
  • Endothelial cell migration is crucial for processes like angiogenesis and wound healing.

Purpose of the Study:

  • To develop a more sensitive method for measuring cell migration.
  • To utilize automated high-content screening for cell migration analysis.
  • To assess the impact of growth factors and chemical compounds on endothelial cell migration.

Main Methods:

  • Combined BD Biosciences FluoroBlok membrane-based Boyden chambers with the Cellomics HCS ArrayScan.
  • Developed a novel assay based on counting nuclei of migrated cells.

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Last Updated: May 12, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
08:26

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors

Published on: September 18, 2013

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
07:07

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Published on: July 10, 2015

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
11:00

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Published on: June 23, 2023

  • Compared the new method to conventional total well fluorescence measurements.

    • Main Results:

    • The new assay demonstrated approximately 10-fold increased signal/background compared to conventional methods.
    • The method accurately quantifies endothelial cell migration in response to growth factors.
    • The assay is suitable for screening chemical compounds for their effects on cell migration.

    Conclusions:

    • The integrated system provides a highly sensitive and robust method for cell migration studies.
    • This approach enhances the ability to investigate endothelial cell behavior.
    • The assay facilitates efficient screening for modulators of growth factor-mediated cell migration.