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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Identifying host pathogenic pathways in bovine digital dermatitis by RNA-Seq analysis.

R A Scholey1, N J Evans, R W Blowey

  • 1Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool Science Park IC2, 146 Brownlow Hill, Liverpool L3 5RF, UK.

Veterinary Journal (London, England : 1997)
|April 11, 2013
PubMed
Summary

Digital dermatitis in dairy cattle involves complex bacterial causes and unknown pathogenesis. This study found increased expression of specific genes related to immune evasion and altered keratin production in affected feet, suggesting novel insights into the disease.

Keywords:
Bovine digital dermatitisKeratinPathway analysisRNA-Seqα2-Macroglobulin-like 1

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Area of Science:

  • Veterinary Medicine
  • Molecular Biology
  • Animal Science

Background:

  • Digital dermatitis is a significant welfare concern in dairy cattle, characterized by painful foot lesions.
  • The precise pathogenesis of digital dermatitis, despite its complex multibacterial etiology, remains poorly understood.

Purpose of the Study:

  • To investigate the gene expression profiles in bovine digital dermatitis lesions compared to healthy tissue.
  • To identify key genes and pathways involved in the pathogenesis of digital dermatitis.

Main Methods:

  • RNA-sequencing (RNA-Seq) technology was employed to compare gene expression in skin biopsies from diseased and healthy bovine feet.
  • Transcriptomic data was mapped to the Btau 4.0 genome for differential gene expression analysis.
  • Pathway analysis was conducted to identify relevant gene networks.

Main Results:

  • Bovine digital dermatitis lesions exhibited increased mRNA expression of α2-macroglobulin-like 1, potentially aiding bacterial immune evasion and survival.
  • Elevated expression of keratin 6A and interleukin 1β mRNA was observed in lesions.
  • Most other keratin and keratin-associated genes showed reduced expression, with limited evidence of local immune response to infection.

Conclusions:

  • Gene expression analysis reveals distinct molecular signatures in digital dermatitis lesions, including upregulation of immune evasion-related genes.
  • Altered keratin expression suggests compromised skin barrier function in affected areas.
  • The findings indicate a limited local immune reaction within the lesions, warranting further investigation into disease mechanisms.