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Analytical and bioanalytical technologies for characterizing antibody-drug conjugates
Stephen C Alley1, Kevin E Anderson
1Seattle Genetics, Inc., 21823 30th Drive SE, Bothell, WA 98021, United States. SAlley@seagen.com
Current Opinion in Chemical Biology
|April 11, 2013
Summary
New assays are crucial for characterizing antibody-drug conjugates (ADCs) due to their complex nature. These advanced methods enable better understanding of ADC properties and in vivo behavior.
Area of Science:
- Biochemistry
- Pharmacology
- Analytical Chemistry
Background:
- Antibody-drug conjugates (ADCs) are complex therapeutics requiring extensive characterization.
- Their heterogeneous and dynamic nature presents unique analytical challenges.
Purpose of the Study:
- To review novel assay technologies for ADC characterization.
- To discuss methods for determining physicochemical properties and pharmacokinetic parameters of ADCs.
Main Methods:
- Literature review of recent advancements in analytical and bioanalytical assays for ADCs.
- Focus on technologies enabling assessment of drug-loading distribution and in vivo exposure.
Main Results:
- Identification of new assay technologies for ADC physiochemical characterization.
- Description of methods for determining ADC pharmacokinetic parameters.
Conclusions:
- Advanced assay technologies are essential for comprehensive ADC characterization.
- These assays facilitate understanding of ADC behavior from conjugation to in vivo response.
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