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Updated: May 12, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
Beyond the Hawthorne effect: reduction of Clostridium difficile environmental contamination through active
Dubert M Guerrero1, Philip C Carling, Lucy A Jury
1University Hospitals of Cleveland Case Medical Center, Cleveland, Ohio, USA.
Abstract:
Education and passive observation resulted in a significant improvement in housekeeper disinfection of nontoxigenic Clostridium difficile spores artificially inoculated onto surfaces in C. difficile infection rooms. A further significant reduction occurred with direct supervision and real-time feedback, suggesting that optimal disinfection is achieved by working closely with housekeepers.
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