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Updated: May 12, 2026

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Published on: August 13, 2015
Lymphocyte-platelet crosstalk in Graves' disease
Boris I Kuznik1, Yuri A Vitkovsky, Olga V Gvozdeva
1Physiology Department (BIK, YAV, OVG, AVS), Chita Medical Academy, Chita, Russia; and Clinical Immunology and Allergy Unit, Medicine B Department (EM), Barzilai Medical Center, Ben Gurion University of the Negev, Ashkelon, Israel.
Objective:
Platelets can modulate lymphocytes' role in the pathophysiology of thyroid autoimmune diseases. The present study was performed to clarify the status of platelet-lymphocyte subpopulations aggregation in circulating blood in patients with Graves' disease (GD).
Methods:
One hundred and fifty patients with GD (GD group) and 45 hyperthyroid patients with toxic multinodular goiter (TMG group) were recruited in the study. Control group consisted 150 healthy subjects. Immunophenotyping of lymphocytes was performed by flow cytometry. Detection of lymphocyte-platelet aggregates (LPAs) was done using light microscope after Ficoll-gradient centrifugation.
Results:
The group of GD patients exhibited reduced CD8 lymphocyte and higher CD19 cell counts compared with TMG group and healthy controls. A greater number of activated CD3, HLA-DR+ lymphocytes were observed in GD than in TMG group and control group. GD group was characterized by lower blood platelet count (232 ± 89 × 10 cells/µL) than TMG group (251 ± 97 × 10 cells/µL; P < 0.05) and control group (262 ± 95 × 10 cells/µL; P < 0.05). In GD group, more platelet-bound lymphocytes (332 ± 91 /µL) were found than that in TMG group (116 ± 67/µL, P < 0.005) and control group (104 ± 58 /µL; P < 0.001).
Conclusions:
GD is associated with higher levels of activated lymphocytes and lymphocyte-platelet aggregates.
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