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CD300c is an activating receptor expressed on human monocytes
Venkateswara R Simhadri1, John L Mariano, Aleksandra Gil-Krzewska
1Laboratory of Molecular and Developmental Immunology, Division of Monoclonal Antibodies, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Human CD300c is an activating receptor found on monocytes. This discovery, aided by a specific antibody, reveals its role in enhancing inflammatory responses and immune cell regulation.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human CD300 molecules are immune cell regulators, primarily on myeloid cells.
- Distinguishing CD300a and CD300c expression on lymphocytes was challenging due to antibody limitations.
Purpose of the Study:
- To identify a specific antibody for CD300c.
- To characterize CD300c expression and function on human immune cells.
Main Methods:
- Developed a specific monoclonal antibody (clone TX45) for CD300c.
- Analyzed CD300c expression on leukocytes and monocyte-derived cells.
- Investigated CD300c regulation by TLR ligands and its functional impact.
Main Results:
- Clone TX45 specifically recognizes human CD300c.
- Monocytes are the primary leukocytes expressing CD300c; expression varies in derived cells.
- CD300c engagement triggers calcium mobilization, CD86 upregulation, and cytokine production.
- CD300c activation enhances lipopolysaccharide-induced inflammatory cytokine release.
Conclusions:
- Human CD300c is an activating receptor expressed on monocytes.
- CD300c plays a significant role in modulating inflammatory responses.
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